Department of Structural Biology
School of Medicine, University of Pittsburgh
Rm 1037 Biomedical Science Tower 3
3501 5th Avenue, Pittsburgh, PA 15260

412-648-9056
412-648-9008
ishima@pitt.edu
Homepage: Visit

When and where you received your PhD
Dept of Chemistry, Kyoto University in Japan.

Research

The major objective of our research has been to determine protein structure and dynamics using nuclear magnetic resonance (NMR); spectroscopy, in order to elucidate protein function particularly relating to protein-ligand interactions.

Publications
(click link in the reference for full pdf of the article)

Ishima, R., Ghirlando, R., Toser, J., Gronenborn, A.M., Torchia, D.A., and Louis, J.M. (2001) Folded monomer of HIV-1 protease, J. Biol. Chem., 276(52), 49110-49116.

Louis, J.M., Ishima, R., Nesheiwat, I., Lynch, S.M., Torchia, D.A. and Gronenborn A.M. (2002) Revisiting Monomeric HIV-1 Protease: Characterization and redesign for improved properties. J. Bio. Chem., 278, 6085-6092.

Kato, K., Yamazaki, T., Louis, J.M., Torchia, D.A., and Ishima, R. (2003) An NMR study of the binding kinetics and the internal dynamics of substrate-bound HIV protease in solution. Protein Sci., 12, 1376-1385.

Ishima, R., Torchia, D.A., Lynch, S.M., Gronenborn, A.M., and Louis, J.M. (2003) Solution structure of the mature HIV-1 protease monomer: Insight into the tertiary fold and stability of a precursor. J. Biol. Chem. 267, 43311-43319.

Ishima, R., Baber, J., Louis, J.M., and Torchia, D.A. (2004) Carbonyl carbon transverse relaxation dispersion experiments and ms-us timescale motion in a protein hydrogen bond network. J. Biomol. NMR, 29, 187-198.

Ishima, R. and Torchia, D. A., (2005) Error estimation and global fitting in transverse-relaxation dispersion experiments to determine chemical-exchange parameters, J. Biomol. NMR, 32, 41-54.

Ishima, R. and Torchia, D. A. (2006) Accuracy of optimized chemical-exchange parameters derived by fitting CPMG R2 dispersion profiles when R20a ‚ R20b, J. Biomol. NMR, 34, 209-219.

Gong, Q. & Ishima, R. (2006) 15N-{1H} NOE experiment at high magnetic field strengths.J. Biomol. NMR, 37, 147-157.

People in the lab

Qinguo Gong - Postdoctoral Fellow
Wazo Myint - Graduate Student
Joseph Walsh - Part-time Research Associate