Jonathan Coleman
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Phone (412) 648-8077
Fax (412) 648-9008

University of Pittsburgh
1050 Biomedical Science Tower 3

 

Jonathan Coleman

 
The aim of my research is to elucidate the molecular function, architecture, and high-affinity drug binding sites of synaptic vesicle transporters in neurons by studying their function using biochemical techniques and determining their structures using single particle cryo-EM. I am particularly interested in understanding the conformational changes and mechanism associated with transport. I have developed methods for large-scale expression, stabilization by drugs, and for the production of antibodies which recognize these transporters. The use of transporter-antibody complexes is essential in order to provide mass and molecular features to assist in cryo-EM reconstructions because these transporters are small membrane proteins which are largely ensconced within membrane. Atomic structures of transporters in complex with therapeutic drugs are essential for the design of better small-molecule therapeutics with higher specificity and fewer side-effects and will also advance efforts toward understanding the function of these transporters.  

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Education & Training

Undergraduate

University of British Columbia
Hon. B.Sc. Biochemistry, 2002-2007

Postgraduate

Aarhus University, Department of Physiology
Visiting student in lab of Dr. Jens Peter Anderson, 2010

University of British Columbia, Department of Biochemistry and Molecular Biology
Ph.D. in lab of Dr. Robert Molday, 2007-2013

Oregon Health & Science University, Vollum Institute
Postdoctoral training in lab of Dr. Eric Gouaux, 2013-2020


Selected Awards and Honors


2018 OHSU School of Medicine Paper of the Month
2016 Finalist for OHSU Postdoctoral Paper of the Year
2014-2016 Banting Postdoctoral Fellowship


Representative Publications


Coleman, J.A.*, Navratna, V.*, Antermite D., Yang, D, Bull, J.A., Gouaux, E. (2020). Chemical and structural investigation of the paroxetine-human serotonin transporter complex. eLife, 9:e56427

 

Coleman, J.A.*, Yang, D.*, Zhao, Z., Wen, P., Yoshioka, C., Tajkhorshid, E., Gouaux, E. (2019). Serotonin transporter–ibogaine complexes illuminate mechanisms of inhibition and transport. Nature 569, 141-145.

 

Coleman, J. A., Gouaux, E. (2018). Structural basis for recognition of diverse antidepressants by the human serotonin transporter. Nat. Struct. Mol. Biol. 25, 170-175.

 

Coleman, J. A., Green, E. M., Gouaux, E. (2016). Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram. J. Vis. Exp., e54792, doi:10.3791/54792.

 

Coleman J.A., Green E.M., Gouaux E. (2016). X-ray structures and mechanism of the human serotonin transporter. Nature 532 (7599), 334-339.

 

Green E.M., Coleman J.A., Gouaux E. (2015). Thermostabilization of the human serotonin transporter in an antidepressant-bound conformation. PloS One 10 (12), e0145688.

 

Vestergaard A.L., Coleman J.A., Lemmin T., Mikkelsen S.A., Molday L.L., Vilsen B., Molday R.S., Dal Peraro M., Peter Andersen J. (2014). Critical roles of isoleucine-364 and adjacent residues in a hydrophobic gate control of phospholipid transport by the mammalian P4-ATPase ATP8A2. Pro. Natl. Acad. Sci. 111 (14), E1334-E1343.

 

Coleman J.A.*, Zhu X.*, Djajadi H.R., Molday L.L, Smith R.S., Libby R.T., John S.W.M., Molday R.S. (2014). Phospholipid flippase ATP8A2 is required for normal visual and auditory function and photoreceptor and spiral ganglion cell survival. J. Cell. Sci. 127 (5), 1138-1149.

 

Coleman J.A., Quazi F., Molday R.S. (2013). Mammalian P(4)-ATPases and ABC transporters and their role in phospholipid transport. Biochim. Biophys. Acta. 1831, 555-74.

 

Coleman J.A., Vestergaard A.L., Molday R.S., Vilsen B., Peter Andersen J. (2012). Critical role of a transmembrane lysine in aminophospholipid transport by mammalian photoreceptor P4-ATPase ATP8A2. Proc. Natl. Acad. Sci. 109, 1449-54.

 

Coleman J.A., Molday RS. (2011). Critical role of the beta-subunit CDC50A in the stable expression, assembly, subcellular localization, and lipid transport activity of the P4-ATPase ATP8A2. J. Biol. Chem. 286, 17205-16.

 

Coleman J.A., Kwok M.C., Molday R.S. (2009). Localization, purification, and functional reconstitution of the P4-ATPase Atp8a2, a phosphatidylserine flippase in photoreceptor disc membranes. J. Biol. Chem. 284, 32670-9. 

  

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